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Judy Northill, Ian Mackay 2020. Wuhan coronavirus (2019-nCoV) real-time RT-PCR ORF1ab 2020 (Wuhan-ORF1ab). protocols.io https://dx.doi.org/10.17504/protocols.io.bbsginbwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bbsginbw
Authors: Judy Northill, Ian Mackay
Group: protocols.io Ambassadors, Public Health Virology, Forensic and Scientific Services
Summary: A real-time RT-PCR to detect the "novel Wuhan" betacoronavirus or Wuhan seafood market pneumonia virus. Based on sequence MN908947 made available by Professor Yong-Zhen Zhang, Fudan University, Shanghai, China. The target region is within the ORF1ab sequence.Not tested on wild-type virus (as of 25Jan2020), it is expected to be capable of only detecting the Wuhan coronavirus.NotesAssay is fully optimised (as of 24Jan2020).A final name for this virus has not been decided (as of 25Jan2020).
Affiliations: Public Health Virology, Forensic and Scientific Services, Public Health Virology, Forensic and Scientific Services
Version: 1
Publication Date: 2020
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