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Aymara Mas, Alba Machado-Lopez, Patricia Escorcia, Carlos Simón 2020. Enzymatic disaggregation of human myometrium for 10X Single Cell RNA-seq. protocols.io https://dx.doi.org/10.17504/protocols.io.bb5miq46Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bb5miq46
Authors: Aymara Mas, Alba Machado-Lopez, Patricia Escorcia, Carlos Simón
Summary: This protocol describes the procedure for dissociating myometrial samples, which is based in an enzymatic disaggregation using Collagenase IV and DNAse I. This protocol is adapted from Mas et al. 2012 Fertil Steril with minor modifications.
Affiliations: Igenomix Foundation, INCLIVA, Valencia, Spain, Igenomix Foundation, INCLIVA, Valencia, Spain, Igenomix Foundation, INCLIVA, Valencia, Spain, Igenomix Foundation, INCLIVA, Valencia, Spain; Valencia University, Valencia, Spain; Harvard University, Boston, USA
Version: 1
Publication Date: 2020
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