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Janet Keast, Peregrine Osborne, Nicole Wiedmann 2020. Immunohistochemical labelling of spinal cord neurons involved in bladder activity. protocols.io https://dx.doi.org/10.17504/protocols.io.bakkicuwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bakkicuw
Authors: Janet Keast, Peregrine Osborne, Nicole Wiedmann
Group: SPARC
Summary: This protocol is used for immunohistochemical visualisation of immediate early gene expression (c-Fos or Egr-1) in cryosections of rat lumbosacral spinal cord. Free-floating sections are processed in a double labelling protocol to distinguish immediate early gene expression in different neurochemical classes of spinal cord neurons:ChAT [choline acetyltransferase]: preganglionic neuronsTH [tyrosine hydroxyls]: dopaminergic neuronsPax2: inhibitory interneurons
RRIDs used: RRID:AB_10609634, RRID:AB_2533990, RRID:AB_2097174, RRID:AB_390204, RRID:AB_11214092
Affiliations: University of Melbourne, University of Melbourne, University of Melbourne
Version: 1
Publication Date: 2020
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