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Janet Keast, Peregrine Osborne 2020. Confocal microscopy and characterization of synaptic boutons associated with ganglion neurons. protocols.io https://dx.doi.org/10.17504/protocols.io.bakdics6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bakdics6
Authors: Janet Keast, Peregrine Osborne
Group: SPARC
Summary: This protocol describes confocal microscopy and image analysis procedures for characterizing neuronal cell bodies and their associated synaptic boutons in thick (50 µm) cryosections. The protocol has been applied to rat pelvic ganglia, where neuronal cell bodies have been identified using immunohistochemical markers of specific neuron populations and/or fluorescent retrograde tracer.
Affiliations: University of Melbourne, University of Melbourne
Version: 1
Publication Date: 2020
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