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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bae6ibhe

Authors: Ronaldo De Carvalho Augusto, Aki MINODA, Christoph Grunau

Summary: We describe here a step-by-step protocol for the generation of sequence-ready libraries for the Assay for Transposase Accessible Chromatin (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size. This makes the method suitable for population epigenomics studies. This protocol is based on the protocol from Corces et al. (2016), Buenrostro et al. (2015) and Nextera DNA Library Prep Kit (2017) with some modifications. Controls must be done without organisms as input.

Associated Publications: Augusto RdC, Rey O, Cosseau C, Chaparro C, Vidal-Dupiol J, Allienne J, Duval D, Pinaud S, Tönges S, Andriantsoa R, Luquet E, Aubret F, Sow MD, David P, Thomson V, Joly D, Lima MG, Federico D, Danchin E, Minoda A, Grunau C, A simple ATAC-seq protocol for population epigenetics. Wellcome Open Research doi: 10.12688/wellcomeopenres.15552.2

Affiliations: LBMC @Ecole Normale Supérieure de Lyon, RIKEN Center for Integrative Medical Sciences, Epigenome Technology Exploration Unit, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan, Univ. Perpignan Via Domitia, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860 Perpignan, France

External URL: https://doi.org/10.12688/wellcomeopenres.15552.2

Version: 1

Publication Date: 2021

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