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Sam Li 2019. Flex-T™ Tetramer and Cell Staining Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.babeiajeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.babeiaje
Authors: Sam Li
Group: BioLegend
Summary: Using UV-induced peptide exchange, MHC/peptide monomers can be generated with conditional Flex-T™ monomers that harbor peptides of interest in their binding grooves. These new MHC monomers are subsequently multimerized using streptavidin-fluorophore conjugates. The resulting Flex-T™ reagents can be used for staining antigen-specific T cells and flow cytometric analysis. In humans, the MHC molecules are called HLA (Human Leukocyte Antigen).
Affiliations: BioLegend
External URL: https://www.biolegend.com/protocols/flex-t-tetramer-preparation-and-flow-cytometry-staining-protocol/4251/
Version: 3
Publication Date: 2019
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