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Bulent Arman Aksoy, Pinar Aksoy, Elinor Gottschalk, Jeff Hammerbacher 2020. Measuring relative reactivity of mouse TCRs against a mouse cancer cell line. protocols.io https://dx.doi.org/10.17504/protocols.io.ba8gihtwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.ba8gihtw
Authors: Bulent Arman Aksoy, Pinar Aksoy, Elinor Gottschalk, Jeff Hammerbacher
Group: Hammer Lab
Summary: This protocol repurposes Promega's T Cell Activiation Bioassay workflow to be able to test relative mouse TCR reactivity against a cell line. This specific protocol uses MC38 as the target as it doesn't normally present SIINFEKL and have good H2Kb and H2Db expression levels. The reactivity will be in relative to the positive control (OT-I reactivity against SIINFEKL-pulsed cells) and the negative control (OT-I reactiviy against unpulsed cells).
Affiliations: Medical University of South Carolina, Medical University of South Carolina, Medical University of South Carolina, Medical University of South Carolina
Version: 1
Publication Date: 2020
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