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URL: https://dx.doi.org/DOI:10.17504/protocols.io.9t7h6rn

Authors: Thorsten Langner, Adeline Harant, Sophien Kamoun

Summary: Fungal genomes are highly dynamic and often contain supernumerary mini-chromosomes. However, our knowledge about the role of mini-chromosomes in dynamic genome rearrangements and evolution is scarce. Mini-chromosomes are usually smaller then core-chromosomes and are variable in size and numbers between individuals of a species. Mini-chromosome occurence in fungi is well documented since decades, but many genomic projects have neglected them, mainly because it was technically challenging to identify them in whole genome assemblies. Here we present a protocol for isolation of mini-chromosomes for enrichment sequencing. This allows identification of mini-chromosomes in whole genome assemblies and thus comparative genomics analyses of core- and mini-chromosomes. .justify:after { content: ""; display:inline-block; width: 100%; }

Associated Publications: Langner T, Harant A, Gomez-Luciano LB, Shrestha RK, Malmgren A, Latorre SM, Burbano HA, Win J, Kamoun S (2021) Genomic rearrangements generate hypervariable mini-chromosomes in host-specific isolates of the blast fungus. PLoS Genet 17(2): e1009386. doi: 10.1371/journal.pgen.1009386

Affiliations: The Sainsbury Laboratory, University of East Anglia, Norwich, UK, The Sainsbury Laboratory, University of East Anglia, Norwich, UK, The Sainsbury Laboratory, University of East Anglia, Norwich, UK

External URL: https://doi.org/10.1371/journal.pgen.1009386

Version: 1

Publication Date: 2019

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