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URL: https://dx.doi.org/DOI:10.17504/protocols.io.8vjhw4n

Authors: Angelica S. Gouveia, Lucas L. Lima, Flaviane S. Coutinho, Juliano M. Rodrigues, Valquiria J. M. Pinheiro, Maria Eduarda S. Ramos, Camilo E. Vital, Claudia S.L. Pontes, Daniella P. Pinheiro, Pedro M. Vidigal, Edvaldo Barros, Humberto J O Ramos

Group: Metabolomics Protocols & Workflows

Summary: Plants produce a variety of compounds with diverse structure and abundance that play an important role in the development, growth and response to the environment. Metabolomics involves studies of a great number of metabolites, which are small molecules present in biological systems.Metabolite profiles can be obtained from several samples by LC/MS (liquid chromatography coupled to mass spectrometry). However, the large number of ions detected for each run from different treatments and replicates require automatic processing for both spectra (metabolite identification and quantification) and global analysis of the metabolome. Thus, this protocol describes in detail, step-by-step, the comparison of the metabolite profiles from LC/MS data for determination of dysregulated pathways, as well as for Exploratory Statistical and Functional Enrichment Analyzes. . .justify:after { content: ""; display:inline-block; width: 100%; }

Affiliations: Department of Biochemistry and Molecular Biology - Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP - Viçosa-MG Brazil, Department of Biochemistry and Molecular Biology - Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP - Viçosa-MG Brazil, Department of Biochemistry and Molecular Biology - Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP - Viçosa-MG Brazil, Department of Biochemistry and Molecular Biology - Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP - Viçosa-MG Brazil, Department of Biochemistry and Molecular Biology - Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP - Viçosa-MG Brazil, Department of Biochemistry and Molecular Biology - Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP - Viçosa-MG Brazil, Department of Biochemistry and Molecular Biology - Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP - Viçosa-MG Brazil, Center for Biomolecules Analysis (NuBioMol) Universidade Federal de Viçosa - UFV Viçosa-MG Brazil, Center for Biomolecules Analysis (NuBioMol) Universidade Federal de Viçosa - UFV Viçosa-MG Brazil, Center for Biomolecules Analysis (NuBioMol) Universidade Federal de Viçosa - UFV Viçosa-MG Brazil, Center for Biomolecules Analysis (NuBioMol) Universidade Federal de Viçosa - UFV Viçosa-MG Brazil, Department of Biochemistry and Molecular Biology - Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP - Viçosa-MG Brazil

Version: 2

Publication Date: 2019

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