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Protocol Name
Plasmid: NK588 (pAct-nanoluc)
DOI:DOI:10.17504/protocols.io.8uuhwww RRID Copied  
PDF Report How to cite
David Booth, Nicole King 2019. Plasmid: NK588 (pAct-nanoluc). protocols.io https://dx.doi.org/10.17504/protocols.io.8uuhwww
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.8uuhwww

Authors: David Booth, Nicole King

Group: King Lab

Summary: The attached file is the full sequence for a plasmid that uses gene regulatory elements from S. rosetta to drive the expression of nanoluc luciferase upon transfection in S. rosetta. The plasmid is backbone is pUC19 and can be propagated in E. coli with Ampicillin selection.

Affiliations: University of California, San Francisco, University of California, Berkeley; Howard Hughes Medical Institute

External URL: https://www.addgene.org/109077/

Version: 1

Publication Date: 2019

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