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Manuela De Las Casas 2019. Quantitative paper ELONA. protocols.io https://dx.doi.org/10.17504/protocols.io.8hdht26Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.8hdht26
Authors: Manuela De Las Casas
Group: AEGIS - Madrid iGEM 2019
Summary: The aim of this protocol is to check the efficiency of the detection system that will be present in the strips. Like the qualitative assay, this is a dot blot performed with streptavidin and aptamers, also named paper ELONA.The expected result is a good binding between the streptavidin and the aptamers (knowing already that the streptavidin binds correctly to the nitrocellulose membrane thanks to previous experiments), great hybridization between both aptamers and a clear visible result when yielding with the ABTS. In order to make this experiment quantitative, several concentrations are made from the same mix, to test the minimum concentration with a detectable change of color.
Affiliations: Universidad Complutense de Madrid, AEGIS - Madrid iGEM 2019
Version: 2
Publication Date: 2019
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