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URL: https://dx.doi.org/DOI:10.17504/protocols.io.8h6ht9e

Authors: Manuela De Las Casas, Laura Sánchez

Group: AEGIS - Madrid iGEM 2019

Summary: The aim of this protocol is to separate the aptamers that bind to the cells from the ones that don't. The cells used have a histidin tag that attaches to the beads' streptavidin tag. The beads will then pull the cells and the complex will precipitate. Since the aptamers have high specifity and affinity for particular sequences, those able to recognice the cells will bind to them, being part of the complex. The aptamers that don't recognice the cells, won't bind. This protocol enables to separate aptamers based on their affinity using the target cells.The Dynabeads His-Tag Isolation and Pulldown from ThermoFisher is an already proven system to separate and isolate proteins. We have automatised the system with the Opentrons to separate cells and it worked succesfully. But if you don't have an Opentrons machine, it is also possible to perform a manual protocol as described here.

Affiliations: Universidad Complutense de Madrid, AEGIS - Madrid iGEM 2019, Universidad Complutense de Madrid, AEGIS - Madrid iGEM 2019

Version: 1

Publication Date: 2019

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