Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/DOI:10.17504/protocols.io.8fkhtkw

Authors: Sandra Rieger

Group: Diabetic Complications Consortium

Summary: To induce hyperglycemia in larval zebrafish, utilize a genetic β-cell ablation system involving the Tg(ins:NTR-mCherry) transgenic zebrafish strain (courtesy of Dr. Michael Parsons, Johns Hopkins University). This strain expresses the bacterial enzyme Nitroreductase (NTR), which is encoded by the nfsB gene, in β-cells of the pancreas starting around 24 hours post fertilization (hpf). Because NTR is genetically fused to the red fluorescent protein mCherry, β-cells expressing NTR can be visualized with fluorescence microscopy. Upon addition of the prodrug metronidazole (MTZ) to the embryo medium, NTR converts MTZ into a toxin, which selectively ablates NTR-expressing β-cells (Curado et al., Nature Protocols 3, 948-954 (2008). This can be monitored by the disappearance of the fluorescence under a confocal microscope. Diabetic Complication:

Affiliations: University of Miami

External URL: https://www.diacomp.org/shared/document.aspx?id=221&docType=Protocol

Version: 2

Publication Date: 2019

Expand All Collapse