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Alba Balletbó, Sebastiaan Kuiper 2019. Agarose Gel Electrophoresis. protocols.io https://dx.doi.org/10.17504/protocols.io.7zmhp46Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.7zmhp46
Authors: Alba Balletbó, Sebastiaan Kuiper
Summary: Gel electrophoresis is the standard lab procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively charged DNA through an agarose gel matrix toward a positive electrode. Shorter DNA fragments migrate through the gel more quickly than longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an agarose gel alongside a DNA ladder (a collection of DNA fragments of known lengths).
Affiliations: Wageningen University, Wageningen University
Version: 1
Publication Date: 2019
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