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Guillermo Fernández Rodríguez 2019. Cell-ELONA. protocols.io https://dx.doi.org/10.17504/protocols.io.7umhnu6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.7umhnu6
Authors: Guillermo Fernández Rodríguez
Group: AEGIS - Madrid iGEM 2019
Summary: ELONA (Enzyme-Linked Oligonucleotide Assay), is a biochemical method based on enzyme linked immunosorbent assay (ELISA) that allows to demonstrate that aptamers selected by SELEX can be effective and useful as biorecognition molecules and laboratory tools. The ELONA format choosed uses an anti-digoxigenin antibody to recognize an aptamer previously labelled with digoxigenin. This antibody is conjugated with a peroxidase enzyme, and once it adds ABTS solution, it will be responsible for the colourimetric reaction which will be detected. (We used 5 replicates per dilution)
Affiliations: AEGIS - Madrid iGEM 2019
Version: 1
Publication Date: 2019
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