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Ji Gao 2019. Measurement of XylE (Catechol 2,3-Dioxygenase) enzyme activity by microplate reader. protocols.io https://dx.doi.org/10.17504/protocols.io.7suhnewCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.7suhnew
Authors: Ji Gao
Summary: Simple measurement of XylE (Catechol 2,3-Dioxygenase) enzyme activity by microplate reader. Catechol 2,3-Dioxygenase can catalyze catechol (1,2-Dihydroxybenzene) to 2-HMS, which has a high absorbance at 377 nm, so we may use photometric mode of microplate reader to measure the concentration of 2-HMS, product of the enzyme.
Affiliations: Tsinghua University
Version: 1
Publication Date: 2019
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