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New England Biolabs 2020. PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): Amplification of bisulfite-converted, deaminated, or damaged DNA (Including FFPE DNA). protocols.io https://dx.doi.org/10.17504/protocols.io.7sdhna6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.7sdhna6
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5®High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
Affiliations: New England Biolabs
External URL: https://www.neb.com/protocols/2019/07/02/pcr-using-q5u-hot-start-high-fidelity-dna-polymerase-neb-m0515
Version: 1
Publication Date: 2020
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