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URL: https://dx.doi.org/DOI:10.17504/protocols.io.7r4hm8w

Authors: New England Biolabs

Group: New England Biolabs (NEB)

Summary: Rapid and irreversible heat inactivation eliminates unwanted activityImproved storage stability versus native enzymeFaster reaction setup (no supplemental additives like zinc required) and shorter incubation timeFlexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisRecombinant for purity, consistency and value

Affiliations: New England Biolabs

External URL: https://neb.com/protocols/2019/06/04/enzymatic-pcr-cleanup-protocol-neb-m0525

Version: 1

Publication Date: 2020

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