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Brittany Zepernick, Matthew Saxton, Steven Wilhelm 2019. Quantifying Biogenic Silica (bSi) Deposition Rates Adapted Method & Fluorescence Reading (PDMPO) via Fluorometer. protocols.io https://dx.doi.org/10.17504/protocols.io.735hqq6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.735hqq6
Authors: Brittany Zepernick, Matthew Saxton, Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Great Lakes Center for Fresh Waters and Human Health
Summary: This method can be used to assess and quantify the rate of silica deposition (bSi) over time in diatoms to determine their rate of frustule synthesis. This protocol has been adapted for the processing of both cultures as well as environmental samples when inoculated with PDMPO [2‐(4‐pyridyl)‐5‐((4‐(2dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole], which is a fluorescent dye that is co-deposited with silica during frustule biosynthesis in a 3230:1 Si:PDMPO (mol:mol) ratio.
Affiliations: University of Tennessee, Knoxville, Miami University of Ohio, The University of Tennessee, Knoxville
External URL: http://wilhelmlab.utk.edu/
Version: 1
Publication Date: 2019
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