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URL: https://dx.doi.org/DOI:10.17504/protocols.io.72mhqc6

Authors: Alexandra Ehl, David Frommholz, Nadine Stefanczyk

Summary: Purification Guide for the Isolation of Antibodies with ChroPack Columns by DALEX Biotech.With ChroPack Protein A columns you can quickly isolate monoclonal and polyclonal antibodies from various sample materials. Antibodies bind with high specificity to the recombinant protein A whose BSA binding site has been removed. The ChroPack columns can be operated with peristaltic pumps and common FPLC systems. The Luer-Lock connections make changing the column particularly easy.One-time autoclavable with DALEX stabilizer for Protein A.Each ml bed volume has a binding capacity of > 10 mg (tested with polyclonal human Ig, the binding capacity varies according to species and clones).Up to 98 % purity in a single purification step.No swelling/shrinking of the matrix in aqueous buffers.High thermal stability, up to 15 minutes at 80 °C in aqueous buffers with neutral pH.pH stability: short-term pH 2 - 8, long-term pH 3 - 8.Suitable for all common low-pressure systems.

Affiliations: DALEX Biotech, DALEX Biotech, DALEX Biotech

Version: 1

Publication Date: 2019

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