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DOI:DOI:10.17504/protocols.io.6wchfaw RRID Copied  
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Xiaohua Du, Xia Liu, Mawolo James Blackar, Yingjie Zhou, Haifeng Wang, Fayang Liu, Zhiqing He 2019. Spectrophotometry method for the detection of Biochemical parameters. protocols.io https://dx.doi.org/10.17504/protocols.io.6wchfaw
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URL: https://dx.doi.org/DOI:10.17504/protocols.io.6wchfaw

Authors: Xiaohua Du, Xia Liu, Mawolo James Blackar, Yingjie Zhou, Haifeng Wang, Fayang Liu, Zhiqing He

Summary: Definition of key termsSpectrophotometry is an experimental technique that is used to measure the concentration of solutes in a specific solution by calculating the amount of light absorbed by those solutes.[1] This technique is powerful because certain compounds will absorb different wavelengths of light at different intensities. By analyzing the light that passes through the solution, you can identify particular dissolved substances in solution and how concentrated those substances are. A automatic analyser (7180) is the device used to analyze solutions in a laboratory research setting.Desecription of Automatic analyserThe Automatic analyser (7180) is a medium-sized biochemistry automatic analyzer.The minimum required amount of reaction solution is smaller due to a new optical design, and running cost is lower and working efficiency higher because the operating unit is usable in the same way as a general-purpose personal computer. Further, this analyzer features a wide range of applications including routine, stat and special analyses.Benefit of Spectrophotometry methodThe usage of Automatic analyser (Hitachi Ltd 7180 Serial, Tokyo, Japan) enabled us to obtained more samples result in less time and this prevented other factors from influencing our tested results.

Associated Publications: Liu X, Mawolo JB, Du X, Zhou Y, Wang H, Liu F, He Z, Marela HA (2019) Investigation of biochemical and physiological parameters of the newborn Saiga antelope (Saiga tatarica) in Gansu Province, China. PLoS ONE 14(11): e0224822. doi: 10.1371/journal.pone.0224822

Affiliations: Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou City, Gansu Province, People's Republic of China, College of Life Science and Technology, Gansu Agricultural University, Lanzhou City, Gansu Province, People's Republic of China, College of Life Science and Technology, Gansu Agricultural University, Lanzhou City, Gansu Province, People's Republic of China , Gansu Endangered Animal Protection Center of State Forestry Administration, Wuwei City, Gansu Province, People's Republic of China, College of Life Science and Technology, Gansu Agricultural University, Lanzhou City, Gansu Province, People's Republic of China , College of Life Science and Technology, Gansu Agricultural University, Lanzhou City, Gansu Province, People's Republic of China, Gansu Endangered Animal Protection Center of State Forestry Administration, Wuwei City, Gansu Province, People's Republic of China

External URL: https://doi.org/10.1371/journal.pone.0224822

Version: 1

Publication Date: 2019

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