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Marcos Otero-Garcia, Inma Cobos 2019. Isolation of single nuclei from postmortem fresh frozen human brain and immunostaining for NeuN. protocols.io https://dx.doi.org/10.17504/protocols.io.6tuhenwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.6tuhenw
Authors: Marcos Otero-Garcia, Inma Cobos
Group: Neurodegeneration Method Development Community
Summary: - Protocol based on Krishnaswami et al., Nat Protoc. 2016, 3:499-524- Used routinely in our lab for the isolation of single nuclei from fresh frozen postmortem brains from subjects with neurodegenerative disorders and healthy controls for single-nucleus RNA-seq- Works also for isolation of nuclei from fresh frozen mouse brains - Used for isolation of all nuclei or antibody-enriched populations (i.e., NeuN+ nuclei)- We always perform and strongly recommend FACS if used for single-nucleus RNA-seq
Affiliations: Stanford University, Stanford University
Version: 1
Publication Date: 2019
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