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Elizabeth Jannaman, Peter Hansen 2019. Zona pellucida removal using Acid Tyrode solution and snap freezing. protocols.io https://dx.doi.org/10.17504/protocols.io.6kwhcxeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.6kwhcxe
Authors: Elizabeth Jannaman, Peter Hansen
Group: University of Florida Bovine Embryo Lab
Summary: A simple protocol to remove the zona pellucida from bovine embryos. This is a necessary step before freezing embryos for analysis of RNA or DNA because of contaminating cumulus and sperm cells associaed with the zona pellucida.
Affiliations: University of Florida, University of Florida
External URL: https://animal.ifas.ufl.edu/hansen/lab_protocol_docs/Acidtyrodes.pdf
Version: 1
Publication Date: 2019
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