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URL: https://dx.doi.org/DOI:10.17504/protocols.io.6f2hbqe

Authors: Khairil Asnan, Mohd. Sadzan Asram bin Aliming, Aprialdy Idrus, Diana Eka Pratiwi

Summary: Cattle's blood component is compressed using centrifuge (5000 rpm for 10 minutes), then supernatant (erythrocytes) collected. Erythrocytes with a ratio of 3:1 (serum:supernatant), treated with initial mixing with NaOH (0.5 M) with an initial ratio 1:1 (v/v) and let it sit for 30 seconds then mixed it by centrifugation about 10 rpm for 30 seconds. Sample then treated by mixing oleic acid (2:1 v/v). The sample then dehydrated by heat about 121oC for a week. Calculated total iron mass was 240000 μg/100 ml whole blood sample (about 14.40% content of the whole sample). Sample purified by furnace using high temperature about 800oC for 2 hours and increased the iron concentration up to 46.30% (m/m%). .justify:after { content: ""; display:inline-block; width: 100%; }

Affiliations: Biology Department, Universitas Negeri Makassar, Chemistry Department, Universitas Negeri Makassar, Chemistry Department, Universitas Negeri Makassar, Chemistry Department, Universitas Negeri Makassar

Version: 2

Publication Date: 2019

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