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URL: https://dx.doi.org/DOI:10.17504/protocols.io.69qhh5w

Authors: Tonis Org, Kati Hensen, Rita Kreevan, Elina Mark, Olav Sarv, Reidar Andreson, Ülle Jaakma, Andres Salumets, Ants Kurg

Summary: Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) has revolutionized our understanding of chromatin-related biological processes. The method, however, requires thousands of cells and has therefore limited applications in situations where cell numbers are limited. Here we describe a novel method called Restriction Assisted Tagmentation Chromatin Immunoprecipitation (RAT-ChIP) that enables global histone modification profiling from as few as 100 cells. The method is simple, cost-effective and takes a single day to complete.

Associated Publications: Org T, Hensen K, Kreevan R, Mark E, Sarv O, Andreson R, Jaakma

Affiliations: Department of Biotechnology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia, Department of Biotechnology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia, Department of Biotechnology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia, Department of Reproductive Biology, Estonian University of Life Sciences, Tartu, Estonia., Competence Centre on Health Technologies, Tartu, Estonia, Department of Bioinformatics, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia; Institute of Genomics, University of Tartu, Tartu, Estonia, Department of Reproductive Biology, Estonian University of Life Sciences, Tartu, Estonia., Competence Centre on Health Technologies, Tartu, Estonia; Department of Obstetrics and Gynaecology, Institute of Clinical Medicine, University of Tartu, Tartu, Estonia; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia; Department of Obstetrics and Gynaecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland., Department of Biotechnology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia

External URL: https://doi.org/10.1371/journal.pone.0225801

Version: 1

Publication Date: 2019

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