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Mark Moosburner, Andrew Allen 2019. CRISPR-Cas9 episome conjugation into Phaeodactylum tricornutum. protocols.io https://dx.doi.org/10.17504/protocols.io.5pvg5n6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.5pvg5n6
Authors: Mark Moosburner, Andrew Allen
Group: A.E. Allen Lab
Summary: Bacterial-conjugation methodology used to introduce the CRISPR-Cas9 episome generated using the GG2 assembly protocol.This method, though, can be adapted for any episomal transfromation from an E. coli culture that contains a cargo plasmid (episome) and conjugation plasmid (pta-MOB) to Phaeodactylum.
Associated Publications: Moosburner MA, Gholami P, McCarthy JK, Tan M, Bielinski VA, Allen AE, Multiplexed Knockouts in the Model Diatom by Episomal Delivery of a Selectable Cas9. Frontiers in Microbiology doi: 10.3389/fmicb.2020.00005
Affiliations: University of California, San Diego, University of California, San Diego
External URL: https://doi.org/10.3389/fmicb.2020.00005
Version: 2
Publication Date: 2019
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