Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Lisa Sudek, Alexandra Worden, Manny Ares 2019. Reporter titrations for transfection of Micromonas commoda CCMP2709. protocols.io https://dx.doi.org/10.17504/protocols.io.57rg9m6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.57rg9m6
Authors: Lisa Sudek, Alexandra Worden, Manny Ares
Group: Protist Research to Optimize Tools in Genetics (PROT-G), Worden Lab
Summary: This protocol describes the method used to test various amounts (0, 0.3, 3, 10, 20 and 30 µg) of reporter (H3proMpo-LUC) DNA. The plasmid H3proMpo-LUC carries a codon optimized Nanoluc coding region, whose expression is driven by the histone H3 promoter and 3' mRNA end formation and termination sequences. Lumen per total number of cells (including transfected and non-transfected cells within a culture) is used as a proxy for relative transfection efficiencies. It is determined in each culture based on luminescence measurements performed 72 hrs after transfection using the Nano-Glo® Luciferase Assay System.
Affiliations: Monterey Bay Aquarium Research Institute, Moss Landing, USA, Monterey Bay Aquarium Research Institute, Moss Landing, USA; Ocean EcoSystems Biology Unit, Marine Ecology Division, Helmholtz Centre for Ocean Research, Kiel, Germany, University of California, Santa Cruz
Version: 1
Publication Date: 2019
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
