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Protocol Name
TRIO tracing
DOI:DOI:10.17504/protocols.io.4xsgxne RRID Copied  
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Hongwei Dong 2019. TRIO tracing. protocols.io https://dx.doi.org/10.17504/protocols.io.4xsgxne
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.4xsgxne

Authors: Hongwei Dong

Summary: TRIO (Tracing the Relationship of Inputs and Outputs) experiments systematically inject complementary viruses into different target brain regions to monosynaptically label inputs to a specific projection neuron population. TRIO experiments use 3 different viruses (AAVretro-Ef1a-Cre, AAV8-hSyn-FLEX- TVA-P2A-GFP-2A-oG helper virus, and EnvA pseudotyped G-deleted Rabies mCherry (EnvA RVdG-4mCherry).

Affiliations: USC Mark and Mary Stevens Neuroimaging and Informatics Institute

External URL: https://cic.ini.usc.edu/protocols/trio-tracing

Version: 1

Publication Date: 2019

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