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Marta Tomaselli, Marius Rebmann 2019. Marchantia high throughput imaging in multiwell plates. protocols.io https://dx.doi.org/10.17504/protocols.io.4v4gw8wCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.4v4gw8w
Authors: Marta Tomaselli, Marius Rebmann
Group: OpenPlant Project
Summary: This protocol allows high throughput imaging of Marchantia gemmae, using a cheap setup made with broadly available lab equipment. We used a transparent 384 wells plate filled with 1/2 strength Gamborg B5 media with 1.2% agar and placed a single gemma at the centre of the well. Gene frames and coverslips treated with anti-fog spray were used to cover (seal) the wells. This setup can be paired with automated imaging of samples.
Affiliations: University of Cambridge, Open Plant, Plant Sciences, University of Cambridge, OpenPlant
Version: 1
Publication Date: 2019
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