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URL: https://dx.doi.org/DOI:10.17504/protocols.io.4uagwse

Authors: Stemcell Technologies

Group: STEMCELL Technologies

Summary: Multipotent neural progenitor cells (NPCs) generate the major cell types of the central nervous system (CNS): neurons, astrocytes and oligodendrocytes. Human pluripotent stem cells (hPSCs), including embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, can be directed to differentiate into NPCs using a variety of methods. This process, known as ‘neural induction’, must be efficient and reliable, in order to generate high-quality NPCs for downstream applications. Although neural induction of hPSCs using embryoid body (EB) formation allows for visual assessment of induction success via the formation of neural rosettes, the whole process can take anywhere between 16 and 19 days to get single-cell neural progenitor cells (NPCs). As a result, monolayer culture-based neural induction methods have recently gained popularity, since they enable single-cell NPCs to be obtained in as few as six days. Here we describe a procedure for neural induction using STEMdiff™ Neural Induction Medium in a monolayer culture-based system to efficiently generate PAX6-positive NPCs.

Affiliations: STEMCELL Technologies

External URL: https://www.stemcell.com/monolayer-culture-method-neural-induction-hpscs-lp.html?utm_source=protocolsio&utm_medium=referral

Version: 1

Publication Date: 2019

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