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URL: https://dx.doi.org/DOI:10.17504/protocols.io.4rfgv3n

Authors: Marc T. J. Johnson, Eric J. Carpenter, Zhijian Tian, Richard Bruskiewich, Jason N. Burris, Charlotte T. Carrigan, Mark W. Chase, Neil D. Clarke, Sarah Covshoff, Claude W. dePamphilis, Patrick P. Edger, Falicia Goh, Sean Graham, Stephan Greiner, Julian M. Hibberd, Ingrid Jordon-Thaden, Toni M. Kutchan, James Leebens-Mack, Michael Melkonian, Nicholas Miles, Henrietta Myburg, Jordan Patterson, J. Chris Pires, Paula Ralph, Megan Rolf, Rowan F. Sage, Douglas Soltis, Pamela Soltis, Dennis Stevenson, C. Neal Stewart Jr, Barbara Surek, Christina J. M. Thomsen, Juan Carlos Villarreal, Xiaolei Wu, Yong Zhang, Michael K. Deyholos, Gane Ka-Shu Wong

Summary: This protocol is part of a collection of eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation from Plant Tissue Collection: https://www.protocols.io/view/rna-isolation-from-plant-tissue-439gyr6) and was originally published as part of Appendix S1 of "Evaluating Methods for Isolating Total RNA and Predicting the Success of Sequencing Phylogenetically Diverse Plant Transcriptomes" Marc T. J. Johnson et al. PLOS ONE, November 21, 2012. https://doi.org/10.1371/journal.pone.0050226

Affiliations: Department of Biology‚ University of Toronto at Mississauga‚ Mississauga‚ Ontario‚ Canada, Department of Biological Sciences‚ University of Alberta‚ Edmonton‚ Alberta‚ Canada, BGI-Shenzhen‚ Bei Shan Industrial Zone‚ Yantian District‚ Shenzhen‚ China, International Rice Research Institute‚ Metro Manila‚ Philippines, Department of Plant Sciences‚ University of Tennessee‚ Knoxville‚ Tennessee‚ United States of America, Department of Plant Biology‚ University of Georgia‚ Athens‚ Georgia‚ United States of America, Jodrell Laboratory‚ Royal Botanic Gardens‚ Kew‚ Richmond‚ Surrey‚ United Kingdom, Genome Institute of Singapore‚ Singapore‚ Singapore, Department of Plant Sciences‚ University of Cambridge‚ Cambridge‚ United Kingdom, Department of Biology and Intercollege Graduate Program in Plant Biology‚ Huck Institutes of the Life Sciences‚ Pennsylvania State University‚ University Park‚ Pennsylvania‚ United States of America, Division of Biological Sciences‚ University of Missouri‚ Columbia‚ Missouri‚ United States of America, Genome Institute of Singapore‚ Singapore‚ Singapore, Department of Botany and UBC Botanical Garden‚ University of British Columbia‚ Vancouver‚ British Columbia‚ Canada, Max Planck Institute for Molecular Plant Physiology‚ Wissenschaftspark Golm‚ Am Mühlenberg 1‚ Potsdam-Golm‚ Germany, Department of Plant Sciences‚ University of Cambridge‚ Cambridge‚ United Kingdom, Department of Biology‚ University of Florida‚ Gainesville‚ Florida‚ United States of America; Florida Museum of Natural History‚ University of Florida‚ Gainesville‚ Florida‚ United States of America, Donald Danforth Plant Science Center‚ St. Louis‚ Missouri‚ United States of America, Department of Plant Biology‚ University of Georgia‚ Athens‚ Georgia‚ United States of America, Department of Botany‚ Cologne Biocenter‚ University of Cologne‚ Cologne‚ Germany, Department of Biology‚ University of Florida‚ Gainesville‚ Florida‚ United States of America; Florida Museum of Natural History‚ University of Florida‚ Gainesville‚ Florida‚ United States of America, Department of Plant Biology‚ North Carolina State University‚ Raleigh‚ North Carolina‚ United States of America, Department of Biological Sciences‚ University of Alberta‚ Edmonton‚ Alberta‚ Canada, Division of Biological Sciences‚ University of Missouri‚ Columbia‚ Missouri‚ United States of America, Department of Biology and Intercollege Graduate Program in Plant Biology‚ Huck Institutes of the Life Sciences‚ Pennsylvania State University‚ University Park‚ Pennsylvania‚ United States of America, Donald Danforth Plant Science Center‚ St. Louis‚ Missouri‚ United States of America, Department of Ecology and Evolutionary Biology‚ University of Toronto‚ Toronto‚ Ontario‚ Canada, Department of Biology‚ University of Florida‚ Gainesville‚ Florida‚ United States of America, Florida Museum of Natural History‚ University of Florida‚ Gainesville‚ Florida‚ United States of America, New York Botanical Garden‚ Bronx‚ New York‚ United States of America, Department of Plant Sciences‚ University of Tennessee‚ Knoxville‚ Tennessee‚ United States of America, Department of Botany‚ Cologne Biocenter‚ University of Cologne‚ Cologne‚ Germany, Department of Biology‚ University of Toronto at Mississauga‚ Mississauga‚ Ontario‚ Canada, Department of Ecology and Evolutionary Biology‚ University of Connecticut‚ Storrs‚ Connecticut‚ United States of America, BGI-Shenzhen‚ Bei Shan Industrial Zone‚ Yantian District‚ Shenzhen‚ China, BGI-Shenzhen‚ Bei Shan Industrial Zone‚ Yantian District‚ Shenzhen‚ China, Department of Biological Sciences‚ University of Alberta‚ Edmonton‚ Alberta‚ Canada, Department of Biological Sciences‚ University of Alberta‚ Edmonton‚ Alberta‚ Canada; BGI-Shenzhen‚ Bei Shan Industrial Zone‚ Yantian District‚ Shenzhen‚ China; Department of Medicine‚ University of Alberta‚ Edmonton‚ Alberta‚ Canada

Version: 1

Publication Date: 2019

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