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Arnav Moudgil, Rob Mitra 2019. Processing Single Cell Calling Card Sequencing Data. protocols.io https://dx.doi.org/10.17504/protocols.io.4phgvj6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.4phgvj6
Authors: Arnav Moudgil, Rob Mitra
Group: Transposon Calling Cards
Summary: Here we present a computational pipeline for processing single cell calling card (scCC) data. These data will have been generated from single cell RNA-seq libraries following transfection/-duction of either undirected piggyBac transposase or your favorite transcription factor (YFTF) fused to piggyBac. This workflow demonstrates how to process scCC sequencing data derived from a 10x Chromium-based scCC library; the workflow can be parallelized on distributed computing architectures (e.g. slurm).
Affiliations: Washington University, Saint Louis, Washington University, Saint Louis
Version: 1
Publication Date: 2019
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