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Ben Matthews 2019. Transposase injection mix protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.4a6gsheCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.4a6gshe
Authors: Ben Matthews
Summary: This protocol details the steps necessary to generate injection mixes (comprised of plasmid DNA for integration and transposase mRNA) for transposon-mediated integration into Aedes aegypti and other insects. It has been empirically tested in Ae. aegypti embryos with piggyBac transposase. In our hands, using mRNA as a source of transposase instead of a helper plasmid increases transformation rates substantially.
Affiliations: University of British Columbia
Version: 1
Publication Date: 2019
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