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Robert Auber, Jennifer Wisecaver 2019. Algal DNA extraction for HMW Nanopore sequencing. protocols.io https://dx.doi.org/10.17504/protocols.io.45dgy26Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.45dgy26
Authors: Robert Auber, Jennifer Wisecaver
Group: High molecular weight DNA extraction from all kingdoms, Wisecaver Lab
Summary: This is a protocol adapted for a haptophte algae. It is a single cellular organism lacking any type of silica or calcium carbonate armor. If your organism doesn't lyse by a simple snap freeze, some type of grinding step is recommended. Confirmation of properly extracting the nuclei can be done with propidium iodide staining and an epiflourescent microscope. The actual DNA extraction gives ultra long reads (greater than 200kb) by using a circulomics NanoBind kit.
Affiliations: Purdue University, Purdue University
Version: 1
Publication Date: 2019
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