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Lev Tsypin, Aaron Turkewitz 2019. Cell deposition and drug sensitivity assay. protocols.io https://dx.doi.org/10.17504/protocols.io.336gqreCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.336gqre
Authors: Lev Tsypin, Aaron Turkewitz
Group: Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: This protocol describes how to deposit several cells of S. robusta in droplets on a petri dish, allowing the experimenter to track the growth of approximately single cell isolates with high throughput. This was used to determine drug sensitivity.We found that in, liquid culture, S. robusta is sensitive to 10 ug/mL puromycin and 250 ug/mL glyphosate. One caveat to keep in mind is that S. robusta appears more tolerant to antibiotics when grown on agar plates, being insensitive to up to 20 ug/mL of puromycin, for example. We recommend selection in liquid culture.
Affiliations: California Institute of Technology, University of Chicago
Version: 1
Publication Date: 2019
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