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URL: https://dx.doi.org/DOI:10.17504/protocols.io.32qgqdw

Authors: Meriem Bekliz, Hannes Peter

Summary: This series of sequential protocols can be used to extract and purify viruses from stream biofilm samples for metagenomic analysis. We compared each critical step of the protocol to alternatives and evaluated their performance using epifluorescence counting of viral-like particles and DNA yields. The pipeline consists of concentration of sample using tangential flow filtration, extraction using tetrasodium pyrophosphate and sonication and purfication using DNase I treatment, filtration, and ultracentrifugation in a sucrose density gradient. Where appropriate, we provide links to similar protocols.

Associated Publications: Bekliz M, Brandani J, Bourquin M, Battin TJ, Peter H, Benchmarking protocols for the metagenomic analysis of stream biofilm viromes. PeerJ doi: 10.7717/peerj.8187

Affiliations: Stream Biofilm and Ecosystem Research Laboratory, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland, EPFL - EPF Lausanne

External URL: https://doi.org/10.7717/peerj.8187

Version: 3

Publication Date: 2019

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