Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Laura Bortolin, Melissa Goldman, Steven Mccarroll 2020. Extraction of Nuclei from Brain Tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.2srged6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.2srged6
Authors: Laura Bortolin, Melissa Goldman, Steven Mccarroll
Group: McCarroll Lab
Summary: This protocol enables rapid isolation of intact nuclei from brain tissue, including highly myelinated adult brain tissue. We have used it successfully to extract nuclei for single-nucleus RNA-seq from human, mouse, marmoset, and macaque brain.
Affiliations: Harvard Medical School; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Harvard Medical School; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Harvard Medical School; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard
Version: 1
Publication Date: 2020
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
