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Protocol Name
DOI:DOI:10.17504/protocols.io.23fggjn RRID Copied  
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Renuka Kudva, Andreas Vogt, Kärt Denks, Gunnar von Heijne 2019. Isolation of ribosome-associated nascent chains of soluble proteins produced in Escherichia coli. protocols.io https://dx.doi.org/10.17504/protocols.io.23fggjn
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.23fggjn

Authors: Renuka Kudva, Andreas Vogt, Kärt Denks, Gunnar von Heijne

Summary: Isolation of ribosome-nascent chains of co-translationally folded domains. Detailed description of protocol published in https://doi.org/10.1073/pnas.1810523115. The arrest sequence used for stalling was TnaC (the leader peptide of the tryptophanase operon).

Affiliations: Stockholm University, Stockholm University, Max Planck Institute for Biophysical Chemistry, Stockholm University

Version: 1

Publication Date: 2019

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