Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/10.17504/protocols.io.zmff43n

Authors: Laura Poza-Viejo, Ivan del Olmo, Pedro Crevillén

Summary: Chromatin Immunoprecipitation (ChIP) is a crucial technique to study chromatin regulation, epigenetic phenomena and transcription factor DNA-binding in vivo. This technique is widely used in model plant systems like Arabidopsis but reliability in other plant systems is challenging. We adapted our well-established Arabidopsis ChIP protocol to be used with leaves and inflorescences of Brassica crops species (Brassica rapa and Brassica napus). This protocol was originally based on Gendrel et al. Nature Methods volume 2, pages 213–218 (2005), but has several modifications inlcluding chromatin sonication using a BioRuptor (Diagenode), immunoprecipitation using magnetic Dynabeads (Thermofisher) and DNA elution recovery performed using Chelex 100 (Bio Rad). The protocol is quick and very reproducible and has been validated against a number of histone modifications and protein tag antibodies. .justify:after { content: ""; display:inline-block; width: 100%; }

Affiliations: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28223 Pozuelo de Alarcón (Madrid), Spain, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28223 Pozuelo de Alarcón (Madrid), Spain, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28223 Pozuelo de Alarcón (Madrid), Spain

Version: 1

Publication Date: 2019

Expand All Collapse