Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Brenton Paolella 2019. Cell Hashing. protocols.io dx.doi.org/10.17504/protocols.io.yp6fvreCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.yp6fvre
Authors: Brenton Paolella
Summary: This protocol is for performing Cell Hashing only. Sample multiplexing and super-loading on single cell RNA-sequencing platforms.Cell Hashing uses a series of oligo-tagged antibodies against ubiquitously expressed surface proteins with different barcodes to uniquely label cells from distinct samples, which can be subsequently pooled in one scRNA-seq run. By sequencing these tags alongside the cellular transcriptome, we can assign each cell to its sample of origin, and robustly identify doublets originating from multiple samples.
Affiliations: Broad Institute
External URL: https://cite-seq.com/cell-hashing/
Version: 2
Publication Date: 2019
Expand AllComing soon.
Check
for all resource mentions.
Coming soon.
Source: Protocols.io
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
