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Ian Mackay, Judy Northill 2019. Enterovirus (EV) A71 real-time RT-PCR (EV-A71-TM2018). protocols.io https://dx.doi.org/10.17504/protocols.io.xb3fiqnCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.xb3fiqn
Authors: Ian Mackay, Judy Northill
Group: Public Health Virology, Forensic and Scientific Services
Summary: This protocol aims to amplify enterovirus (EV) A71 viruses but not other viruses.This protocol was designed by us.The oligonucleotides target the 5'UTR noncoding region. This is a qualitative test for investigating EV-A71 infection of humans.The test has identified both historical EV-A71 strains and contemporary strains and has confirmed these using subgenomic sequencing of partial 5'UTR-VP2 and partial VP1 sequencing. Culture of the virus is not required as this assay is capable of detecting EV-A71 direct from extracted clinical samples.
Affiliations: Public Health Virology, Forensic and Scientific Services, Public Health Virology, Forensic and Scientific Services
Version: 2
Publication Date: 2019
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