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Vincent Bielinski, Chris Dupont 2018. Modified HMW DNA Isolation from Stramenopiles with Agar Plugs. protocols.io dx.doi.org/10.17504/protocols.io.wenfbdeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.wenfbde
Authors: Vincent Bielinski, Chris Dupont
Group: Protist Research to Optimize Tools in Genetics (PROT-G), JCVI West Protocols
Summary: This protocol is the adaptation of previously published methods for use in isolating intact high molecular weight DNA from stramenopiles for long-read sequencing. The advantages here are minimal amount of centrifugation steps on isolated DNA, as well as the long-term storage of the DNA while in plugs to allow experimental flexibility.
Affiliations: J. Craig Venter Institute, Synthetic Biology & Bioenergy Group, J. Craig Venter Institute, Synthetic Biology & Bioenergy Group
Version: 1
Publication Date: 2018
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Source: Protocols.io
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