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Janet Keast, Peregrine Osborne 2019. Immunohistochemical classification of sensory and autonomic neurons projecting to the lower urinary tract in rats [keast-001]. protocols.io dx.doi.org/10.17504/protocols.io.w3gfgjwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.w3gfgjw
Authors: Janet Keast, Peregrine Osborne
Group: SPARC
Summary: This collection describes the procedures required to label, visualise, characterise and quantify neurons that innervate the lower urinary tract tissues of adult male and female Sprague-Dawley rats. This collection includes protocols for:STAGE 1: Surgery to micro-inject fluorescent retrograde tracer dyes into one or more sites within the lower urinary tractSTAGE 2: Intracardiac perfusion with fixative to preserve neural tissues of interestSTAGE 3: Fluorescence immunohistochemistry of ganglion cryosections.
Affiliations: University of Melbourne, University of Melbourne
Version: 1
Publication Date: 2019
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Source: Protocols.io
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