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Atsuko Mizoguchi 2018. Purification of total RNA (microRNA and mRNA) from liquid samples. protocols.io dx.doi.org/10.17504/protocols.io.vu3e6ynCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.vu3e6yn
Authors: Atsuko Mizoguchi
Summary: Total RNA was extracted from cells and the cultured supernatant and exosome which was extracted from the same amount of supernatant using 3D-Gene® RNA extraction reagent.Comprehensive miRNA and mRNA expression analysis were performed using 3D-Gene® Human miRNA Oligo Chip (miRBase ver.21) and mRNA Oligo Chip(Toray Industries, Inc.) which are featured with the columnar structure and bead-mixing for high sensitivity.
Associated Publications: Mizoguchi A, Takayama A, Arai T, Kawauchi J, Sudo H (2018) MicroRNA-8073: Tumor suppressor and potential therapeutic treatment. PLoS ONE 13(12): e0209750. doi: 10.1371/journal.pone.0209750
Affiliations: toray industries Inc.,
External URL: https://doi.org/10.1371/journal.pone.0209750
Version: 1
Publication Date: 2018
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Source: Protocols.io
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