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Martin Thomas Jahn 2019. Isolate prokaryotes from sponge tissue (SAP). protocols.io dx.doi.org/10.17504/protocols.io.u8vezw6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.u8vezw6
Authors: Martin Thomas Jahn
Group: hostmicrobeprotocols
Summary: Protocoll to create a fixed suspension of sponge associated prokaryotes (SAP) from sponge tissue.This can serve as the basis for Fluorescence in situ hybridisation and/or cell sorting.This protocol was tested for different sponge species but might also be adapted to other organisms (let others know)The protocol was modified from :Fieseler L, Horn M, Wagner M, Hentschel U. (2006) Discovery of the novel candidate phylum "Potibactetia" in marine sponges (vol 70, pg 3724, 2004). Applied and Environmental Microbiology;72(8):5677-.PMID:15184179 DOI:10.1128/AEM.70.6.3724-3732.2004
Affiliations: CRC1182
Version: 1
Publication Date: 2019
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Source: Protocols.io
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