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Shuntai Zhou 2018. Primer ID MiSeq Library Prep. protocols.io https://dx.doi.org/10.17504/protocols.io.sjdeci6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.sjdeci6
Authors: Shuntai Zhou
Summary: This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed adapters with KAPA2G Robust and KAPA HiFi PCR kits, respectively.
Affiliations: University of North Carolina at Chapel Hill
Version: 3
Publication Date: 2018
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