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Harley King 2020. Using a Peristaltic Pump to Flow Buffer Through a Nanoporous Membrane in Filter Holder Assembly. protocols.io dx.doi.org/10.17504/protocols.io.rwqd7dwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.rwqd7dw
Authors: Harley King
Summary: This protocol provides steps for seating and flowing liquid through a 50 μm, 13mm round membrane containing a hexagonal arrangement of 25nm pores with lattice constant 65nm using a peristaltic pump. Porous, alumina membranes are fragile and pressure is required to pass buffer through the 25nm pores.A peristaltic pump at a low flow rate reproducibly hydrates membranes compared to syringe-based assemblies. Steps in this protocol minimize membrane breaks and damage during membrane placement in the filter adapter assembly, liquid flow through the membrane and membrane retrieval.
Affiliations: LuminUltra
Version: 1
Publication Date: 2020
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Source: Protocols.io
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