Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Stephen Floor 2018. Around-the-horn PCR and cloning. protocols.io dx.doi.org/10.17504/protocols.io.rf2d3qeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.rf2d3qe
Authors: Stephen Floor
Group: Stephen Floor Lab
Summary: This protocol is designed for 'around-the-horn' or 'divergent' PCR, where primers go around most or all of a plasmid but are pointed away from each other so they generate a linear product. Note that this protocol is written for Q5 polymerase, but works fine with other polymerases. To switch polymerases, just change the PCR reaction setup.
Affiliations: UCSF
Version: 1
Publication Date: 2018
Expand AllComing soon.
Check
for all resource mentions.
Coming soon.
Source: Protocols.io
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
