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Protocol Name
Second-derivative UV Spectroscopy
DOI:10.17504/protocols.io.q7qdzmw RRID Copied  
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Moriah Beck 2019. Second-derivative UV Spectroscopy. protocols.io dx.doi.org/10.17504/protocols.io.q7qdzmw
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.q7qdzmw

Authors: Moriah Beck

Group: Beck Lab

Summary: Second-derivative spectroscopy has proven to be an effective analytical tool because of its ability to resolve overlapping bands in the normal spectrum.This protocol is used to determine the degree of tyrosine exposure in proteins using second-derivative UV spectroscopy.

Affiliations: Wichita State University

Version: 1

Publication Date: 2019

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Source: Protocols.io