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Andrew Potter 2018. Adult mouse lung cell dissociation (on ice). protocols.io dx.doi.org/10.17504/protocols.io.q6bdzanCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.q6bdzan
Authors: Andrew Potter
Group: Human Cell Atlas Method Development Community
Summary: This protocol was used to dissociate adult (8-10 wk) mouse lung tissue. The entire procedure is carried out on ice (to reduce artifact gene expression changes) and takes about half an hour. The yield was 16,240 non-RBC/mg tissue with 87% viability.
Affiliations: University of Cincinnati
Version: 1
Publication Date: 2018
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Source: Protocols.io
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